Generation of CYP3A4-expressing human ES/iPS cell-derived hepatocytes for drug screening

2016 Fiscal Year Final Research Report

• Project/Area Number: 15H06365
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Medical pharmacy
• Research Institution: Osaka University
• Principal Investigator: Takayama Kazuo 大阪大学, 薬学研究科, 特任助教(常勤) (10759509)
• Project Period (FY): 2015-08-28 – 2017-03-31
• Keywords: 肝細胞 / ヒトES/iPS細胞 / 薬物代謝 / CYP / 肝毒性 / 創薬試験

Outline of Final Research Achievements

In this study, we aimed to establish an efficient differentiation method for highly functioning hepatocyte-like cells from human ES/iPS cells. First, we attempted to improve the hepatocyte differentiation method. Next, we developed a method to concentrate the highly functioning hepatocyte-like cells. The hepatic functions of human ES/iPS cell-derived hepatocyte-like cells were enhanced by culturing with conditioned medium of hepatocytes and cholangiocytes. In future, our group is going to establish the genetically engineered human ES/iPS cells, which carry puromycin resistant cassette under the CYP3A4.

Free Research Field

医療系薬学