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2016 Fiscal Year Final Research Report

Streptococcus pyogenes endopeptidase O contributes to evasion from complement-mediated bacteriolysis via human complement factor C1q

Research Project

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Project/Area Number 15H06380
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Pathobiological dentistry/Dental radiology
Research InstitutionOsaka University

Principal Investigator

Ogawa Mariko  大阪大学, 歯学研究科, 特任研究員 (20754732)

Project Period (FY) 2015-08-28 – 2017-03-31
Keywords化膿レンサ球菌 / 補体 / エンドペプチダーゼ / 古典経路 / C1q
Outline of Final Research Achievements

In this study, we identified an endopeptidase of Streptococcus pyogenes, PepO, as an interacting molecule with complement C1q, and investigated its effects on complement immunity and pathogenesis.
ELISA results revealed that PepO bound to C1q in a concentration-dependent manner under physiological conditions. Then, the effects of PepO on bacterial evasion from complement immunity was analyzed in various pH condition. Under low pH conditions, PepO inhibited the binding of IgG to C1q. In addition, pepO deletion rendered S. pyogenes more susceptible to the bacteriocidal activity of human serum. The wild-type strain (WT)-infected tissues exhibited greater severity and lower complement activity as compared to those infected by ΔpepO in a mouse skin infection model.
Our results suggest that interaction of S. pyogenes PepO with C1q interferes with the complement pathway, which enables S. pyogenes to evade complement-mediated bacteriolysis under acidic conditions.

Free Research Field

口腔細菌学

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Published: 2018-03-22  

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