Studies of the molecular mechanisms in the regulation of telomerase activity and cell immortarization.

2016 Fiscal Year Final Research Report

• Project/Area Number: 15H06414
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: General medical chemistry
• Research Institution: Tottori University
• Principal Investigator: Takahito Ohira 鳥取大学, 医学(系)研究科(研究院), 助教 (60757665)
• Research Collaborator: Inaoka Daigo ; Kuroda Yuko
• Project Period (FY): 2015-08-28 – 2017-03-31
• Keywords: テロメレース / hTERT / メラノーマ / LC/MS/MS / ＰＩＴＸ１ / 不死化 / がん抑制遺伝子

Outline of Final Research Achievements

Telomerase ribonucleic enzyme plays an essential role in cellular immortalization. The catalytic subunit of telomerase holoenzyme is　hTERT know key regulator of telomerase activity. Thus, hTERT is an attractive target for the discovering of cancer-specific treatment. Previously, we reported PITX1 can suppress hTERT expression for regulation of transcription manner in melanoma cells. However, the detailed mechanism underlying which negative regulation including counterpart factors remains unclear.
Here, using FLAG pull-down methods, we identified ZCCHC10 as a novel PITX1 interacting protein. PITX1 protein interacts via their homeodomain with the CCHC domain of ZCCHC10. Furthermore, co-expression of PITX1 and ZCCHC10 inhibition of hTERT transcription in A2058 cells. Additionally, ZCCHC10 expression level is decrease in melanoma cell lines and tissues. These data suggested that ZCCHC10-PITX1 complex proteins are a novel regulator of telomerase activity.

Free Research Field

腫瘍学