Elucidation of the role of TFG in glucose and lipid metabolism in pancreas, liver and adipose tissue

2016 Fiscal Year Final Research Report

• Project/Area Number: 15H06427
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Metabolomics
• Research Institution: Hiroshima University
• Principal Investigator: Yamamotoya Takeshi 広島大学, 医歯薬保健学研究院(医), 助教 (50760013)
• Project Period (FY): 2015-08-28 – 2017-03-31
• Keywords: 糖尿病 / インスリン分泌 / TFG

Outline of Final Research Achievements

To elucidate the role of TFG in insulin secretion, we generated pancreatic β-cell specific TFG knockout mice (βTFG KO) and analyzed the phenotype. βTFG KO displayed marked hyperglycemia with impaired insulin secretion on glucose tolerance test. Impairment of both β-cell mass and function was suggested by smaller insulin-positive area in the immunohistochemical analysis of the pancreas and reduced insulin secretion on glucose stimulation in isolated islets. Electron microscopic observation and microarray gene expression analysis revealed smaller insulin crystal diameters, mild ER stress and downregulation of Nrf2 and its downstream genes as possible underlying mechanisms for the impaired β-cell mass and function in βTFG KO.

Free Research Field

糖尿病・代謝