Study of the reaction between beta-lactam antibiotids and thiol compounds

2016 Fiscal Year Final Research Report

• Project/Area Number: 15H06509
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Bacteriology (including mycology)
• Research Institution: Kumamoto University
• Principal Investigator: Ono Katsuhiko 熊本大学, 大学院生命科学研究部(医), 助教 (80573592)
• Project Period (FY): 2015-08-28 – 2017-03-31
• Keywords: βラクタム系抗生物質 / システイン / チオール化合物

Outline of Final Research Achievements

Recently we found that beta-lactam antibiotics is inactivated by reaction with thiol compounds such as cysteine. On the basis of LC-MSMS and NMR analysis, it was shown that beta-lactam antibiotics and cysteine formed one to one adduct. E.coli fliY mutant, which is loss of cysteine/cystien shuttle system, showed high-sensitive to beta-lactam antibiotics and produced less cysteine-beta lactic antibiotics adduct compared with wild type strain. These results suggest that bacterial cysteine might contribute to antibiotics resistance via formation of cysteine-beta-lactam antibiotics adduct in periplasmic space.

Free Research Field

分子生物学