2016 Fiscal Year Annual Research Report

Multiple Endodermal Organoid Generation from Robustly Amplified Human Posterior Gut Progenitors

Research Project

Project/Area Number	15H06534
Research Institution	Yokohama City University
Principal Investigator	張冉冉横浜市立大学, 医学研究科, 博士研究員 (10760887)
Project Period (FY)	2015-08-28 – 2017-03-31
Keywords	PGEC / liver bud / transplantation / pancreas
Outline of Annual Research Achievements	In our research, we demonstrated the generation of human CDX2+ posterior gut endoderm cells (PGECs) from induced pluripotent stem cell (iPSC) by manipulating FGF, TGF and Wnt signaling pathways. By screening the gut elongation factors, combinatorial EGF, VEGF, FGF2, Chir99021 and A83-01 treatments amplify storable PGECs over a number of passages up to 1021 cells. PGECs, showed more stable differentiation propensity into liver, pancreas and gut lineage cells both in vitro and in vivo. Human PGECs liver bud organoids, showed therapeutic potential against fulminant liver failure upon transplantation. Together, the use of PGECs, as an alternative to PSC, may be a stable cellular source of endoderm-derived organoids for studying human development, modeling disease and ultimately therapy.
Research Progress Status	28年度が最終年度であるため、記入しない。
Strategy for Future Research Activity	28年度が最終年度であるため、記入しない。