2016 Fiscal Year Final Research Report
Functional analysis of docosahexaenoic acids in late-endosome specific phospholipid BMP
| Project/Area Number |
15H06600
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Biological pharmacy
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| Research Institution | Juntendo University |
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Principal Investigator |
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| Project Period (FY) |
2015-08-28 – 2017-03-31
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| Keywords | ドコサヘキサエン酸 / リン脂質 / 後期エンドソーム / BMP / 脂質 / DHA / DPAn-6 |
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| Outline of Final Research Achievements |
BMP is an enigmatic phospholipid that is specifically localized in intralumenal vesicles of late endosomes, and is thought to be essential for proper functions of late endosomes. BMP has a propensity to incorporate an omega-3 fatty acid DHA, and DHA is highly enriched in BMP. However, the physiological meaning of this DHA-containing BMP fatty acyl species is unknown, mainly due to the fact that biosynthetic enzymes of BMP have not been identified so far.
A non-targeted lipidomic analysis on knockout mouse organs of a lipid metabolizing enzyme recently identified a sought-after enzyme for the biosynthesis of BMP. The knockout organs exhibited reduced levels of DHA-containing BMP species. Furthermore, in testes from a recently generated PUFA-deficient mouse model, DPAn-6, which usually constitutes more than 70% of fatty acids in BMP, was nearly depleted. These mouse models seem to be very useful to reveal physiological roles of the unique fatty acyl composition of BMP.
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| Free Research Field |
脂質生化学
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