Development of an analytical method for trace elemental analysis in single particle/single cell

2016 Fiscal Year Final Research Report

• Project/Area Number: 15H06768
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Analytical chemistry
• Research Institution: Kwansei Gakuin University
• Principal Investigator: Iwai Takahiro 関西学院大学, 理工学部, 助教 (90756694)
• Research Collaborator: CHIBA Koichi ; OKINO Akitoshi ; INAGAKI Kazumi
• Project Period (FY): 2015-08-28 – 2017-03-31
• Keywords: 単一細胞分析 / 微小量試料分析 / 微量元素分析

Outline of Final Research Achievements

To analyze trace elements in single cell and single particle, an ICP analytical system was established by hyphenating droplet direct injection nebulizer, desolvation system and ICP spectrometer. we succeeded to increase mass signal intensity of Mg in single algae cell 30 times by optimizing sample heating region in desolvation system. To evaluate spectral analysis precision of the system, uniform-sized particles of magnetic polymer latex were analyzed and 19.5% of relative standard deviation was obtained from this system. Single human cancer cells were introduced into this system and emission spectra of Mg and Ca, which is contained in single cell pg level, were successfully detected simultaneously.

Free Research Field

分析化学