The Development of Novel Protein Intracellular Affinity Pairs for Fabrication of Designer Metabolons.

2016 Fiscal Year Final Research Report

• Project/Area Number: 15H06859
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Biofunction/Bioprocess
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Mori Yutaro 国立研究開発法人理化学研究所, 環境資源科学研究センター, 特別研究員 (50758539)
• Project Period (FY): 2015-08-28 – 2017-03-31
• Keywords: Protein Design

Outline of Final Research Achievements

By assembling metabolic enzymes that continuously perform reactions inside E. coli, this study aims to construct a system that improves efficiency of production of valuable chemicals being targeted. As a preliminary step toward that, the applicant developed and designed new protein affinity pairs that exhibit binding affinity within E. coli cells. In this study we achieved the protein expression of affinity pairs in E. coli, and we confirmed that the protein pairs shows the binding affinity in vitro assay.

Free Research Field

Enzyme Engineering