Development of a technique to recover the decreased ability of spermatogenesis

2016 Fiscal Year Final Research Report

• Project/Area Number: 15H06864
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Integrative animal science
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Suzuki Shinnosuke 国立研究開発法人理化学研究所, バイオリソースセンター, 基礎科学特別研究員 (00755994)
• Project Period (FY): 2015-08-28 – 2017-03-31
• Keywords: 精子形成

Outline of Final Research Achievements

In this study, I focused on the differential expression patterns between germ line stem cells (GSCs) and defective GSCs (dGSCs), but I can not find a core factor of keeping the spermatogenic ability. On the other hand, I try to establish a new technique to regulate the multiple gene expression using modified genome editing technologies, CRISPR/Cas9 system, because the genes which expression are dramatically reduced in dGS cells compared to GS cells have the multiple loci and its function analysis is difficult.

Free Research Field

生殖細胞