ベクターコントロールによる有機塩素系農薬汚染が家畜・ヒトに及ぼす毒性影響の解明

2017 Fiscal Year Annual Research Report

• Project/Area Number: 15J02013
• Research Institution: Hokkaido University
• Principal Investigator: Thompson Lesa 北海道大学, 獣医学研究科, 特別研究員(DC1)
• Project Period (FY): 2015-04-24 – 2018-03-31
• Keywords: DDT / Chicken / Transcriptomics / Metabolomics / MCF-7 cell culture

Outline of Annual Research Achievements

Two manuscripts have been published. Various alterations in mRNA expression were detected in chickens exposed to DDTs in the field, including down-regulation of several avian beta-defensins. A manuscript on these findings is currently at the revision stage of publication.mRNA expression was assessed using qRT-PCR after in vitro exposure of p,p’-DDT in MCF-7 human breast cancer cells. Several genes relating to oxidative stress markers, xenobiotic metabolizing enzymes and transporters were significantly up-regulated after exposure. A manuscript has been submitted for peer-review.Samples from in vivo exposure of chickens to p,p’-DDT underwent further analysis. Liver histopathology did not show significant changes. qRT-PCR of liver samples confirmed altered gene expression in avian beta defensin (AvBD) genes, AvBD1 and AvBD7. Further, metabolomics analysis using hydrophilic interaction liquid chromatography (HILIC) revealed many significantly elevated metabolites relating to amino acid metabolism in exposed groups. These results are currently being prepared for journal submission.

Research Progress Status

29年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

29年度が最終年度であるため、記入しない。

Research Products

• [Int'l Joint Research] North West University/BioAssets Consultants/University of Johannesburg(South Africa)
  • Country Name: South Africa
  • Counterpart Institution: North West University/BioAssets Consultants/University of Johannesburg
• [Int'l Joint Research] Zagazig University(Egypt)
  • Country Name: Egypt
  • Counterpart Institution: Zagazig University
• [Int'l Joint Research] University of Gondar(Ethiopia)
  • Country Name: Ethiopia
  • Counterpart Institution: University of Gondar
• [Int'l Joint Research] University of Edinburgh/University of Glasgow/Chester Zoo(英国)
  • Country Name: UNITED KINGDOM
  • Counterpart Institution: University of Edinburgh/University of Glasgow/Chester Zoo
• [Journal Article] Human Health Risk from Consumption of Marine Fish Contaminated with DDT and Its Metabolites in Maputo Bay, Mozambique (2018)
  • Author(s): Thompson, L.A., Ikenaka, Y., Yohannes, Y.B., Ichise, T., Ito, G., Bortey-Sam, N., van Vuren, J.J., Wepener, V., Smit, N.J., Darwish, W.S. and Nakayama, S.M.M., Ishizuka, M.
  • Journal Title: Bull Environ Contam Toxicol Volume: In press Pages: In press
  • DOI: https://doi.org/10.1007/s00128-018-2323-7
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Concentrations and human health risk assessment of DDT and its metabolites in free-range and commercial chicken products from KwaZulu-Natal, South Africa (2017)
  • Author(s): Thompson L. A.、Ikenaka Y.、Yohannes Y. B.、van Vuren J. J.、Wepener V.、Smit N. J.、Darwish W. S.、Nakayama S. M. M.、Mizukawa H.、Ishizuka M.
  • Journal Title: Food Additives & Contaminants: Part A Volume: 34 Pages: 1959～1969
  • DOI: https://doi.org/10.1080/19440049.2017.1357209
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] β-carotene and retinol reduce benzo[a]pyrene-induced mutagenicity and oxidative stress via transcriptional modulation of xenobiotic metabolizing enzymes in human HepG2 cell line (2017)
  • Author(s): Darwish Wageh Sobhy、Ikenaka Yoshinori、Nakayama Shouta、Mizukawa Hazuki、Thompson Lesa A、Ishizuka Mayumi
  • Journal Title: Environ Sci Pollut Res Int Volume: 25 Pages: 6320～6328
  • DOI: https://doi.org/10.1007/s11356-017-0977-z
  • Peer Reviewed / Int'l Joint Research
• [Presentation] First, do no harm: balancing the benefits of malaria control against the toxic effects of DDT use (2017)
  • Author(s): Thompson, L.A., Ikenaka, Y., Yohannes, Y., Assefa, A., van Vuren, J., Wepener, V., Smit, N., Vlok, W., Darwish, W.S., Nakayama, S., Mizukawa, H., Ishizuka, M.
  • Organizer: 5th Sapporo Summer Seminar for One Health
  • Int'l Joint Research
• [Presentation] Comparison of food products contaminated by DDTs in South Africa and Mozambique (2017)
  • Author(s): Thompson, L.A., Ikenaka, Y., Wepener, V., Ishizuka, M.
  • Organizer: 19th International Conference on One Health
  • Int'l Joint Research
• [Presentation] Effect of DDTs on gallinacins in the avian immune system. (2017)
  • Author(s): Thompson, L.A., Ikenaka, Y., Yohannes, Y., van Vuren, J., Wepener, V., Smit, N., Assefa, A., Darwish, W.S., Nakayama, S., Mizukawa, H., Ishizuka, M.
  • Organizer: The 10th International Meeting of the Asian Society of Conservation Medicine One Health in Asia Pacific
  • Int'l Joint Research
• [Remarks] Vetmed Tox Hokkaido University
  • URL: http://tox.vetmed.hokudai.ac.jp/en/