2016 Fiscal Year Annual Research Report
遺伝子発現に対するＲＮＡ分解および転写後制御の寄与
| Project/Area Number |
15J05075
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| Research Institution | The University of Tokyo |
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Principal Investigator |
前川 翔 東京大学, 新領域創成科学研究科, 特別研究員(DC1)
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| Project Period (FY) |
2015-04-24 – 2018-03-31
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| Keywords | RNA分解 / 転写制御 / ゲノム制御 / 次世代シークエンス |
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| Outline of Annual Research Achievements |
The original proposal was to identify the potential role of RNA decay in mediating changes of the eventual RNA abundance.
In the last fiscal year, I have refined the RNA decay profile of 5-bromouridine immunoprecipitation pulse chase sequencing (BRIC-seq), where I have used thresholds to filter genes to ensure that I compare RNA decay for genes with RNA half-life that can be precisely determined.
I have managed to identify the set of genes where the changes in RNA decay does not result in changes in the eventual RNA abundance, suggesting some form of transcriptional feedback to maintain the level of RNA abundance. Additionally, I have overlaid the ENCODE transcription factor ChIP-seq based binding site data, and I have found several transcription factors that enrich in the promoter regions of these genes, which could explain the feedback mechanisms.
In addition, through the comparison of HIF-1 ChIP-seq data to the RNA-seq and BRIC-seq (RNA decay) data, I have preliminarily found that RNA abundance regulation through HIF-1 and RNA decay are distinct.
Altogether, in this year I have managed to gather enough material to summarise the findings into a journal publication. To have the research published, I will conduct further validations on the transcription factors and RNA decay factors that mediate changes in RNA abundance.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
I was able to identify a set of genes that are regulated by RNA decay, which may have be transcriptionally repressed and that HIF-1 and RNA decay regulation seems to be distinct.
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| Strategy for Future Research Activity |
I will verify the findings on these genes on the transcriptional repression. I will try and find validate the specific transcription factor that is a candidate in repressing the target genes and validate the RNA binding protein that is implicated. This will be done through ChIP-qPCR, RIP-qPCR and possibly Luc Assay to determine the contribution of RNA decay.
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