Design of a next generation thermoresponsive culture system for creating functional cultured hepatic sheet tissues

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K01317
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Tokyo Women's Medical University
• Principal Investigator: Kobayashi Jun 東京女子医科大学, 医学部, 講師 (20385404)
• Co-Investigator(Renkei-kenkyūsha): AKIYAMA Yoshikatsu 東京女子医科大学, 医学部, 講師 (20349640)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 生体材料 / 再生医療 / 組織工学 / 温度応答性高分子 / ヘパリン

Outline of Final Research Achievements

In order to prepare transplantable hepatocyte sheets with maintaining hepatic functions, a thermoresponsive culture system mimicking biological environments was designed. Transplantable hepatocyte sheets could be prepared while maintaining hepatic functions on a heparin-immobilized thermoresponsive surface containing a component of basement membrane such as growth factors. It was also possible to quantitatively evaluate the temperature-dependent affinity between cellular receptor/growth factor/the heparin-immobilized thermoresponsive surfaces. By using flexible basal substrate and microfluidic devices, more advanced hepatocyte sheet tissue culture system would be constructed.

Free Research Field

生体材料