2017 Fiscal Year Final Research Report
Identification of optimized specifications of artificial extracellular environment for stem cell culture and spheroid formation
| Project/Area Number |
15K01318
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Biomedical engineering/Biomaterial science and engineering
|
|---|
| Research Institution | Tokyo University of Pharmacy and Life Science |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2015-04-01 – 2018-03-31
|
| Keywords | ペプチド / キトサン / インテグリン / シンデカン / 受容体間クロストーク / 細胞接着 / 細胞培養基質 |
|---|
| Outline of Final Research Achievements |
Extracellular matrix proteins are important factor to maintain the stem cell culture in vitro. Multi-extracellular matrix derived active peptides immobilized chitosan matrices were developed to construct the cell culture scaffold. We immobilized various integrin binding peptides on chitosan matrix to investigate the integrin-integrin cross-talks between different integrin subtypes using multi-peptide-chitosan matrices. The biological activities of multi integrin binding peptide-chitosan matrices could enhance by the optimized combinations and mixture ratios of different active peptides. Further, fibronectin mimicked multi integrin binding peptide-chitosan matrices could develop using the fibronectin derived peptides. These findings are important to control the biological activities of cell culture scaffold to culture the stem cell.
|
| Free Research Field |
生体材料学
|
