2017 Fiscal Year Final Research Report
Effects of TGF-beta-TIMP1 pathway on white adipose tissue remodeling by obesity and exercise
| Project/Area Number |
15K01626
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Sports science
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| Research Institution | Kyorin University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | 脂肪組織 / 肥満 / 運動トレーニング / リモデリング / 細胞外マトリックス / TGF-β / TIMP1 |
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| Outline of Final Research Achievements |
In the present study, we examined effects of TGF-β and TIMP1 on WAT remodeling by exercise training (TR) and obesity. The mRNA levels of TGF-β and TIMP1 in WAT of obese mice were significantly higher than those in WAT of control mice, and TR significantly attenuated the expression of these genes. The gene expression and the protein production of TIMP1 in 3T3-L1 cells were upregulated by treatment with TGF-β. Moreover, treatment of 3T3-L1 cells with TIMP1 resulted in attenuation of insulin-induced glucose uptake and phosphorylation of Akt. In addition, the reduction of prothymosin α in 3T3-L1 cells resulted in enhanced gene expressions of TIMPs (TIMP1-3) and TGF-β signaling related factors, such as dermatopontin. These results suggest that, TR improves insulin sensitivity by inhibiting the TGF-β-TIMP1 pathway associated with reduction of glucose uptake in WAT.
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| Free Research Field |
スポーツ生化学
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