Establishment of a simultaneous quantification method of stress markers by using mass spectrometry and examination of its usefulness

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K01652
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied health science
• Research Institution: Shimane University
• Principal Investigator: Kazuo Yamada 島根大学, 医学部, 助教 (30240005)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 質量分析 / ストレスマーカー / 疾患モデル動物 / 高血圧自然発症ラット

Outline of Final Research Achievements

We developed a simultaneous quantification method of several stress markers including oxidative stress which is involved in diseases such as diabetes, hypertension, and cancer by using LC/MS/MS. Administration of foods containing 4% or 8% sodium chloride to spontaneously hypertensive rat (SHR) and Wistor kyoto (WKY) rat decreased KYA concentration in both SHR and WKY plasmas, and that of food containing 4% sodium chloride increased SDMA concentration in SHR plasma, suggesting the possibility that KYA and ADMA could be a salt-sensitive stress marker.

Free Research Field

細胞生物学