Molecular mechanisms of the cellular signaling by which inorganic polyphosphate promotes calcification on osteoblastic cell

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K01793
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biomolecular chemistry
• Research Institution: Hokkaido University
• Principal Investigator: Tsutsumi Kaori 北海道大学, 保健科学研究院, 助教 (80344505)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: ポリリン酸 / 石灰化 / ATP / ミトコンドリア

Outline of Final Research Achievements

Inorganic polyphosphate (polyP) is a linear polymer containing tens to hundreds of orthophosphate residues and found in all living organisms from bacteria to mammals. In the present study, we examined the molecular mechanism of polyP to promote calcification on MC3T3-E1 osteoblastic cells. Cells cultured with polyP were strongly stained with Alizarin red. Long-chain length of polyP was the most effective chain length. PolyP-treated cells induced the depletion of cellular ATP contents accompany with ADP conversion though treatment with polyP did not affect cell growth. In the polyP-treated cells, the mitochondria was swollen with low dense observed in electron microscopy. Mitochondrial membrane potential was significantly decreased in polyP-treated cells. These results suggested that the depletion of intracellular ATP and the decrease in mitochondrial membrane potential by polyP treatment would be a trigger to promote a cellular calcification.

Free Research Field

分子生物学