2017 Fiscal Year Final Research Report
Lipid interaction and headgroup conformation of glycosphingolipids in membrane bilayer
| Project/Area Number |
15K01801
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biomolecular chemistry
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | 糖脂質 / 生体膜 / 構造 / NMR / 合成 |
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| Outline of Final Research Achievements |
Glycosphingolipids (GSLs) are the membrane lipid molecules having common ceramide backbone and oligosaccharide headgroups with various size and charge. GSLs have been thought to interact with cholesterol (Cho) and altered the sugar chain conformation to regulate the protein interactions, but detail was elusive. Here, we used biomimetic membranes to analyze GSL-Cho interaction and the headgroup conformation by solid state NMR and fluorescence spectroscopies. Lactosylceramide was found to form rigid cluster through headgroup hydrogen bonding, and the affinity to Cho was weak. Glucosylceramide received some order effect from Cho, but the headgroup conformation was not significantly altered in the presence of Cho.
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| Free Research Field |
生体分子化学
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