Development of cancer cell-targeting off/on type fluorescence probes based on biological molecules.

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K01827
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Chemical biology
• Research Institution: Nagahama Institute of Bio-Science and Technology
• Principal Investigator: Kawai Yasushi 長浜バイオ大学, バイオサイエンス学部, 教授 (20240830)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 蛍光プローブ / 酵素活性検出 / プテリン

Outline of Final Research Achievements

Folate receptor is a target for novel chemotherapeutic agents because of its overexpression on the surface of many epithelial cancer cells. In this research, diaminopteridine as a recognized moiety of folate receptor was developed as a multifunctional fluorescent probe. This probe can control off-on switching of fluorescence by enzyme activity such as glutathion-S-transferase specifically expressed in cancer cell, and also can oxidatively damage a G-rich portion of DNA oligomer by irradiating UV light in its fluorescence on state.

Free Research Field

生物有機化学