2017 Fiscal Year Final Research Report
Cell specific manipulation of plant hormone responses
| Project/Area Number |
15K01828
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Chemical biology
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| Research Institution | Okayama University of Science |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
三井 亮司 岡山理科大学, 理学部, 教授 (60319936)
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| Co-Investigator(Renkei-kenkyūsha) |
Kasahara Hiroyuki 東京農工大学, 農学部, 教授 (00342767)
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| Research Collaborator |
Zhao Yunde カルフォルニア大学, サンディエゴ校, 教授
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | オーキシン / ケミカルバイオロジー / 植物ホルモン |
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| Outline of Final Research Achievements |
Asymmetric auxin distribution plays a crucial role in plant growth and development to adapt the environmental stimulus. The local biosynthesis of IAA and the inactivation and degradation of IAA would also contribute to the modulation of auxin distribution. We present that new auxin application system using inactive auxin-prodrug and its metabolic activation enzyme. The IAA-prodrug activating enzyme (IAP) was cloned and expressed as GFP-IAP fusion protein in E. coli and Arabidopsis thaliana. Transgenic plant expressing GFP-IAP showed auxin-induced phenotypes when IAA-prodrug was applied. This new tools would be useful tools to dissect the spatiotemporal dynamics of IAA distribution leading to cell-specific auxin responses.
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| Free Research Field |
生物有機化学
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