2017 Fiscal Year Final Research Report
Development of efficient synthetic techniques for bioactive compounds using cross-linked enzyme reactors
| Project/Area Number |
15K04639
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Nano/Microsystems
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| Research Institution | Tokai University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | 固定化酵素 / 酵素リアクター / PEGA樹脂 / 酸化酵素 |
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| Outline of Final Research Achievements |
In this study, we prepared enzyme-immobilized microbeads via an enzyme cross-linking reaction. The aggregated enzyme on microbeads (PEGA resins) was based on poly-Lys supported cross-linked enzyme aggregates. Compared with the free enzymes, immobilized enzymes were more stable at high temperatures, in the presence of a chemical denaturant, or in an organic solvent. They were recycled without appreciable loss of activity. Immobilized tyrosinase was applied for L-3,4-dihydroxyphenylalanine (L-DOPA) synthesis. L-DOPA is used to treat Parkinson’s disease. Immobilized tyrosinase catalyzed the conversion of tyrosine to L-DOPA. The result was much better than those reported for batch processes that used tyrosinase immobilized on carrier materials. In addition, immobilized laccase was applied for the degradation of endocrine-disrupting chemicals such as bisphenol A. The degradation efficiency of the immobilized laccase was better than those of conventional bioreactors.
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| Free Research Field |
ナノマイクロシステム
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