2017 Fiscal Year Final Research Report
Development of Highly-sensitive Technique in Microchip Electrophoretic Analysis of Biomolecules
Project/Area Number |
15K05527
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Analytical chemistry
|
Research Institution | Hirosaki University |
Principal Investigator |
|
Project Period (FY) |
2015-04-01 – 2018-03-31
|
Keywords | 分離分析 / マイクロチップ / 電気泳動分析 / オンライン試料濃縮 / 高感度分析 |
Outline of Final Research Achievements |
In microchip electrophoresis, the microanalytical technique for highly efficient preconcentration and separation of biomolecules by the combination of two online sample concentration techniques, large-volume sample stacking with an electroosmotic flow pump (LVSEP) and field-amplified sample injection (FASI). By utilizing the vacuum-drying method for modifying the coating polymers onto the channel surface, it was successfully achieved that analytes injected throughout a microchannel were concentrated by LVSEP, followed by the electrokinetic introduction of concentrated analytes from a sample reservoir by FASI. In the LVSEP-FASI analysis, up to 38,000-fold sensitivity increase was obtained, which was 245-times higher than normal LVSEP. In the analyses of biomolecules, amino acid enantiomers, peptides and amines were enriched with the concentration factors ranging from 120 to 4800.
|
Free Research Field |
分析化学
|