Development and application of phage interfaces for bacteria detection

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K05541
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Analytical chemistry
• Research Institution: Kochi University
• Principal Investigator: Watanabe Shigeru 高知大学, 教育研究部総合科学系複合領域科学部門, 教授 (70253333)
• Co-Investigator(Kenkyū-buntansha): 波多野 慎悟 高知大学, 教育研究部総合科学系複合領域科学部門, 講師 (70397157)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 金ナノ粒子 / ファージ / 細菌 / 暗視野顕微鏡 / 比色検出

Outline of Final Research Achievements

Bacteriophages are viruses that infect only bacterial cells in order to reproduce. Their natural affinity to the host cells can be used to design highly specific tools. We proposed phage-conjugated silica-gold core-shell nanoparticles as plasmon scattering probes for dark-field optical detection of pathogenic bacteria. By electrostatic layer-by-layer deposition on the silica nanoparticles, the phage-conjugated silica-gold core-shell nanoparticles were prepared. The phage-conjugated silica-gold core-shell nanoparticles selectively bound to the surfaces of target bacteria. The nanoparticles-bound bacteria remarkably increased the light scattering intensity. Our result suggests that the pathogenic bacteria can be detected with high selectivity and sensitivity by the dark-filed microscopy technique. Furthermore, a wide variety of bacteria can be rapidly detected by altering a variety of phages immobilized on the nanoparticles.

Free Research Field

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