Preparation of Ti-binding osteogenic protein by using phosphorylated chitin

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K05554
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Bio-related chemistry
• Research Institution: Hokkaido University
• Principal Investigator: KUBOKI Yoshinori 北海道大学, 地球環境科学研究院, 名誉教授 (00014001)
• Co-Investigator(Kenkyū-buntansha): 田中 俊逸 北海道大学, 地球環境科学研究院, 特任教授 (30142194) ; 戸倉 清一 北海道大学, 地球環境科学研究院, 名誉教授 (40000806)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: リン蛋白質 / チタン / 人工骨 / 人工歯根 / キチン / リン酸化キチン / チタン結合性蛋白質 / チタンと骨の結合

Outline of Final Research Achievements

In 2014, we discovered that bone phosphoproteins, which are collectively called SIBLING proteins family, possessed the titanium-binding ability. Furthermore, the titanium implant devices which were coated with these titanium binding proteins (TiBP) induced more than 100 times higher amount of bone than the non-coated control.　Inspired by this discovery, we chemically phosphorylated chitin to see whether the same effect can occur in the phosphorylated chitin (P-chitin). The result was that P-chitin definitely bound with titanium, and P-chitin coated titanium implant induced several times higher amount of bone, when implanted into rat calvaria. We attributed the unique property of P-chitin to the regular arrangement of phosphorylated groups of N-acetyl glucosamine corresponding with regular crystal units of TiO2. Usefulness of the new findings are self-evident for dental and orthopedic implants.

Free Research Field

骨と歯の生化学