Development of photoaffinity selection system for identification of low abundance proteins

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K05559
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Bio-related chemistry
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: Kaori Sakurai 東京農工大学, 工学(系)研究科(研究院), 准教授 (50447512)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: フォトアフィニティープローブ / 生物活性分子 / 結合タンパク質 / 金ナノ粒子 / 結合タンパク質探索法

Outline of Final Research Achievements

Development of efficient target identification methods is important for accelerating the discovery of new biological pathways and drug targets. We have designed and synthesized gold-nanoparticle based affinity labeling probes, which display bioactive ligands and photoreactive groups for catch and release of binding proteins in one-pot. Gold nanoparticles are suitable as probe scaffolds due to its capability to covalently immobilize organic compounds in a multivalent fashion, the water dispersibility and the high density, which enable facie centrifugal separation. We demonstrated that our gold-nanoparticle probes can crosslink the binding proteins of high μM affinity with high selectivity at nM probe concentration.

Free Research Field

生物有機化学・ケミカルバイオロジー