Substrate identification method for protein phosphatases through the induction of enzyme-substrate intermediate

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K05560
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Bio-related chemistry
• Research Institution: Niigata University
• Principal Investigator: Chuman Yoshiro 新潟大学, 自然科学系, 准教授 (40372263)
• Co-Investigator(Renkei-kenkyūsha): Higashimoto Yuichiro 久留米大学, 医学部, 准教授 (40352124)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: ホスファターゼ / リン酸化 / シグナル伝達 / 酵素

Outline of Final Research Achievements

Protein phosphorylation is strictly regulated by protein kinases and protein phosphatases, and disordered regulation of protein phosphorylation often causes serious disease, such as cancer. It is important to identify substrates for Ser/Thr phosphatases to clarify the signal transduction and disease mechanisms, however, there are still no their reliable methods. Here, we developed two novel substrate-identification methods for Ser/Thr phosphatases, one is method termed Phosphorylation Mimic Phage Display (PMPD), to identify substrate for SCP1 phosphatase using peptide phage display libraries with Mg2+ and AlF4- and another is development of substrate trapping mutants for PPM1D phosphatase. These methods can be useful and applicable for other Ser/Thr phosphatases.

Free Research Field

生体関連化学、酵素化学