2017 Fiscal Year Final Research Report
Pseudo-natural RNA structures as promising modules to construct functional RNA nanostructures with geometrical shapes
Project/Area Number |
15K05561
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Bio-related chemistry
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Research Institution | University of Toyama |
Principal Investigator |
Yoshiya Ikawa 富山大学, 大学院理工学研究部(理学), 教授 (70281087)
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Co-Investigator(Renkei-kenkyūsha) |
FURUTA HIROYUKI 九州大学, 大学院工学研究院, 教授 (40244157)
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Project Period (FY) |
2015-04-01 – 2018-03-31
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Keywords | RNAナノテクノロジー / リボザイム / RNA構造 / ナノ集積構造 |
Outline of Final Research Achievements |
RNA is a biopolymer that is attractive for constructing nanoscale objects with complex structures. The 3D structure of a group I (GI) ribozyme has a modular architecture, which can be separated into an activator module and a catalytic module. The fully active ribozyme can be reconstructed by assembling the two separately prepared modules through specific assembly between the two modules. Such non-covalent assembly of the two modules allows the design of 1D-array and polygonal RNA nanostructures. Through rational redesign of the parent GI ribozyme, we constructed variant GI ribozymes as unit RNAs for 1D-arry oligomers and polygonal-shaped oligomers with catalytic activity. Programmed trimerization and tetramerization of the unit RNAs afforded catalytically active nano-sized RNA triangles and squares, the structures of which were directly observed by AFM. We have also seceded in design and construction of RNA nanostructures by modular ribozymes and a fluorescent RNA aptamer.
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Free Research Field |
生体関連化学
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