Control of Differentiation of Mesenchymal Stem Cells Using Novel Hybrid Scaffolds

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K05570
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Bio-related chemistry
• Research Institution: Tokyo University of Science
• Principal Investigator: Iijima Kazutoshi 東京理科大学, 工学部工業化学科, 助教 (30468508)
• Co-Investigator(Renkei-kenkyūsha): HASHIZUME Mineo 東京理科大学, 工学部工業化学科, 教授 (40333330) ; KIYOKAWA Nobutaka 国立成育医療研究センター研究所, 小児血液・腫瘍研究部, 部長 (60195401)
• Research Collaborator: KOMARU Kanae 東京理科大学, 大学院・総合化学研究科 ; SUZUKI Ryo 東京理科大学, 大学院・総合化学研究科 ; KURIKI Izumi 東京理科大学, 大学院・総合化学研究科 ; IIZUKA Ayako 東京理科大学, 工学部工業化学科
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 間葉系幹細胞 / 複合材料 / 多糖 / ヒドロキシアパタイト / 分化 / 再生医療

Outline of Final Research Achievements

The method to coat polystyrene (PS) with hydroxyapatite (HAp) in a simulated body fluid via a protein adsorption layer has been developed. This method can apply to coat the subjects with complex structures and various kinds of proteins were used as protein adsorption layers. The differentiation from mesenchymal stem cells to osteoblasts was promoted on HAp-coated PS compared to PS for cell culture, and differentiation into adipocytes and chondrocytes were suppressed. In the polysaccharide composite films prepared by utilizing the hot press method, adhesion and proliferation of cells depended on the type of anionic polysaccharides of the films. The influence of mechanical physical properties and signals derived from interaction between sugar of polysaccharide and cell receptor was suggested. The possibility of control of differentiation of mesenchymal stem cells depending on the type of anionic polysaccharide was expected.

Free Research Field

生体機能材料