Development of cell-enclosing fiber scaffold for tissue regeneration

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K05697
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Materials/Mechanics of materials
• Research Institution: Doshisha University
• Principal Investigator: MORITA YUSUKE 同志社大学, 生命医科学部, 教授 (80368141)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 細胞封入ファイバー / スキャホールド / アルギン酸ナトリウム / 組織再生 / 湿式紡糸 / 軟骨細胞

Outline of Final Research Achievements

The spinning device was developed to fabricate chondrocytes-enclosing fiber scaffolds. The fiber scaffolds were fabricated by collecting chondrocytes-enclosing sodium alginate fiber in the CaCl2 solution using the winding drum. Chondrocytes were distributed uniformly in the fiber, and the fiber pitch and cell pitch were controlled by changing the winding speed and traverse speed. Collagen was locally existed around cells in the fiber scaffold for low cell density, whereas collagen was existed along the fiber in the fiber scaffold for high cell density. When sodium alginate was removed from the fiber scaffold by EDTA treatment, the fiber structure was maintained by synthesized extracellular matrix in the case of 33.7±15.1 μm of cell pitch.

Free Research Field

機械材料・材料力学