Development of rapid DNA detection method for multiplex virus detection

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K06111
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Measurement engineering
• Research Institution: Kyushu University
• Principal Investigator: Nakano Michihiko 九州大学, システム情報科学研究院, 准教授 (00447856)
• Co-Investigator(Renkei-kenkyūsha): SUEHIRO Junya 九州大学, 大学院システム情報科学研究院, 准教授 (70206382)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: DNA検出 / 誘電泳動 / インピーダンス計測 / ウイルス検査

Outline of Final Research Achievements

Applications of microbeads dielectrophoresis (DEP) based DNA detection method were investigated. (1) DEP change of microbead by DNA attachment is derived from not only electric charges on DNA but also permittivity of DNA, (2) application on food contamination by detecting pig mitochondrial DNA sensitive , (3) quantitative detection of norovirus gene (10 copies/reaction) in comparing with real-time polymerase chain reaction, and (4) determination of DNA length by measuring frequency dependent conductance change of DNA attached microbeads. These results suggest that the DNA detection can be applied to detect multiple virus target genes.

Free Research Field

静電気生物応用