Study for biomineralization of biofilm formed on carbon steel

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K06530
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Metal making/Resorce production engineering
• Research Institution: University of Human Arts and Sciences (2017); Suzuka National College of Technology (2015-2016)
• Principal Investigator: Ikegai Hajime 人間総合科学大学, 人間科学部, 教授(移行) (60184389)
• Co-Investigator(Kenkyū-buntansha): 平井 信充 鈴鹿工業高等専門学校, その他部局等, 准教授 (50294020)
• Co-Investigator(Renkei-kenkyūsha): KANEMATSU Hideyuki 鈴鹿工業高等専門学校, 材料工学科, 教授 (10185952)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 生体鉱物化 / バイオフィルム / 炭素鋼 / 緑膿菌 / 微生物腐食 / 藍鉄鉱

Outline of Final Research Achievements

This study aimed to clarify the mechanism of biomineralization, that is, precipitation of vivianite also known as ferrous phosphate in biofilm (BF) of Pseudomonas aeruginosa PAO1 formed on a carbon steel surface. P. aeruginosa BF created oxygen concentration cells, which ferrous ions eluted from the carbon steel surface (microbiologically influenced corrosion, MIC). Consequently, it appeared that vivianite was formed from ferrous ions and phosphate ions contained in culture medium. Iron concentration accumulated in BF was approximately 13% in comparison with a carbon steel (100%). Analysis of synchrotron radiation computed tomography images showed a number of concave traces, which seemed to indicate MIC, along concave area of BF with bumpy construction. It appears that MIC observed in the present study is different from mechanism of MIC caused by a commonly accepted oxygen concentration cell model.

Free Research Field

微生物学