Quantitative metabolomics methodology using stable isotope-labeled multi-compound cocktails

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K06557
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Reaction engineering/Process system
• Research Institution: Kyushu University
• Principal Investigator: Izumi Yoshihiro 九州大学, 生体防御医学研究所, 准教授 (70622166)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 定量メタボロミクス / 定量リピドミクス / イオンクロマトグラフィー質量分析 / 超臨界流体クロマトグラフィー質量分析 / in vivoラベリング / in vitroラベリング / マトリックスエフェクト

Outline of Final Research Achievements

One of the current problems in metabolomics is the difficulty in integrating data collected on different days, using different equipment and/or facilities. Accordingly, the development of a fundamental methodology that enables quantitative comparison of large scale data is needed for next-generation metabolomics study. In the present study, we established quantitative metabolomics and lipidomics methodologies based on chromatography mass spectrometry and stable isotope-labeled multi-compound cocktails. The quantitative analytical system developed is a potentially useful tool for the integration or sharing of global metabolomics data.

Free Research Field

メタボロミクス