DNA-conjugated stimuli-responsive material for fabrication of molecular recognition gating membrane

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K06576
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biofunction/Bioprocess
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: TAMAKI Takanori 東京工業大学, 科学技術創成研究院, 准教授 (80567438)
• Research Collaborator: KOYANAGI Fusae
• Project Period (FY): 2015-10-21 – 2018-03-31
• Keywords: 分子認識材料 / DNA / 核酸 / アプタマー / 感温性ポリマー / バイオセンサー / G四重鎖

Outline of Final Research Achievements

DNA-conjugated stimuli-responsive materials coordinate the molecular recognition properties of DNA and actuator properties of thermoresponsive polymer, poly(N-isopropylacrylamide) (pNIPAM). In some specific conditions, DNA-pNIPAM changes its dispersion state in response to whether conjugated DNA is single-stranded (ss) or double-stranded (ds). Under the condition where entropic repulsion controls the aggregation behavior of the conjugate, the aggregation was controlled by whether the dangling end of dsDNA had a specific structure (e.g. G-quadruplex) or not. Then, this property was combined with the molecular recognition of a DNA aptamer. The target protein, thrombin, bound to DNA-pNIPAM, and this binding changed the aggregation behavior of the conjugate.

Free Research Field

化学工学