Metabolic Engineering of yeast strains producing higher alcohols based on the precise metabolic characterization

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K06579
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biofunction/Bioprocess
• Research Institution: Osaka University
• Principal Investigator: Matsuda Fumio 大阪大学, 情報科学研究科, 教授 (50462734)
• Co-Investigator(Renkei-kenkyūsha): ISHII Jun 神戸大学, 大学院・科学技術イノベーション研究科, 教授 (40512546)
• Research Collaborator: MORITA Keisuke ; NISHINO Shunsuke ; NOMURA Yuta ; KINOSHITA Shohei
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 酵母 / 高級アルコール / 代謝工学 / メタボローム分析 / プロテオーム解析

Outline of Final Research Achievements

The central carbon metabolism in budding yeast (Saccharomyces cerevisiae) was engineered to improve the production of butanols from biomass. For the purpose, a precise characterization of yeast metabolism was performed by developing a method to determine concentration of intracellular metabolites as well as to estimate levels of Gibb’s free energy change of metabolic reactionss. The results of the precise analysis suggested that an activation of excess NADH consumption was essential with considering intracellular compartmentalization of yeast cells. Based on the findings, the isobutanol production by yeast was improved by an overexpression of mitochondrial pyruvate carrier proteins.

Free Research Field

代謝工学