Establishment of specific sequence mining method for unknown giant gene clusters

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K06583
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biofunction/Bioprocess
• Research Institution: Hiroshima University
• Principal Investigator: Okamura Yoshiko 広島大学, 先端物質科学研究科, 准教授 (80405513)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 遺伝子特異的検出 / 難培養微生物 / メタゲノム / シングルセルゲノミクス

Outline of Final Research Achievements

The methods for drug screening using genomic resources from VBNC (visible but non-culturable) bacteria are demanded. In this study, the method for target-specific detection, RNase H assisted Rolling Circle Amplification, was established toward whole genome sequence mining. Only target gene-expressed bacteria were labeled by fluorescence probe successfully with this method. This method opens the way to single sell genomics and mining of drug discovery target encoded in giant gene cluster.

Free Research Field

微生物ゲノム工学