Construction of cascade reaction system for production of D-psicose and D-allose

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K06584
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biofunction/Bioprocess
• Research Institution: Kagawa University
• Principal Investigator: Morimoto Kenji 香川大学, 国際希少糖研究教育機構, 准教授 (90363184)
• Co-Investigator(Kenkyū-buntansha): 高田 悟郎 香川大学, 国際希少糖研究教育機構, 准教授 (50322722)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 希少糖 / D-プシコース / D-アロース / 一括生産 / ボロン酸分離

Outline of Final Research Achievements

Shinella sp. NN-6 produced L-rhamnose isomerase and ketose 3-epimerase when it cultivated using L-rhamnose as a carbon source. Rare sugars, D-psicose and D-allose were produced from D-fructose by partial purified enzyme. Boronic acid gel separation apparatus was developed to separate them without desalting treatment, suggesting that enzyme reaction and separation process were able to connect for the first time. The purity of D-psicose and D-allose was almost 100%. Furthermore, recovered D-fructose allowed to use enzyme reaction after concentration control. Taking together, we obtain the prospect for the construction of cascade reaction system for rare sugars production.

Free Research Field

生物機能・バイオプロセス