2017 Fiscal Year Final Research Report
Molecular mechanisms behind the expression of difficult-to-express proteins
| Project/Area Number |
15K06585
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | University of Shizuoka |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
ITO Keisuke 静岡県立大学, 食品栄養科学部, 准教授 (40580460)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | protein expression / Recombinant protein / yeast expression system / protein synthesis / molecular biotechnology / bimolecular engineering |
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| Outline of Final Research Achievements |
Our novel expression system, namely high cell-density expression, have achieved successful expression of a certain range of “difficult-to-express” secretory protein while the molecular mechanisms behind it was remained to be studied. In this project, we identified two transcription factors, Adr1 and Ygr067C, as a potential host factor that is responsible for the cell-density-specific transcriptome change, where certain mRNAs of which transcriptional promoter shared typical Adr1p binding sequence. In fact, the yeast strain lacking Ygr067c showed a significant reduction in the cell-density dependent protein expression. The factor that directly allows the host to produce the difficult-to-express protein under the transcription factor is to be sought.
In parallel, we successfully find certain types of rare codons in recombinant gene help co-translational folding of synthesized protein, resulting in increased amount of recombinant protein in active form.
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| Free Research Field |
生物工学
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