2017 Fiscal Year Final Research Report
Novel regenerative therapies based on injury-induced neural stem cells obtained from patients with stroke
| Project/Area Number |
15K06723
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Neurophysiology / General neuroscience
|
|---|
| Research Institution | Hyogo Medical University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
松山 知弘 兵庫医科大学, 医学部, 教授 (10219529)
田中 康恵 兵庫医科大学, 医学部, 助教 (30723499)
吉村 紳一 兵庫医科大学, 医学部, 教授 (40240353)
|
|---|
| Project Period (FY) |
2015-04-01 – 2018-03-31
|
| Keywords | 脳梗塞 / 幹細胞 / 神経再生 |
|---|
| Outline of Final Research Achievements |
Using a mouse model of cerebral infarction, we have previously demonstrated that neural stem/progenitor cells (NSPCs) develop within ischemic areas. The injury/ischemia-induced NSPCs (iNSPCs) likely originate in part from brain pericytes and they have the potential to differentiate into neural lineages. However, it is essential to understand the traits of iNSPCs in human brain for possible applications in stem cell-based therapies for stroke patients. To examine this, putative iNSPCs were extracted from ischemic brain tissue obtained from elderly stroke patients requiring decompressive craniectomy and partial lobectomy for diffuse cerebral infarction. Immunohistochemistry showed that nestin+ iNSPCs were localized near blood vessels within ischemic areas. Isolated iNSPCs also expressed nestin and demonstrated high proliferative potential. Furthermore, they formed neurosphere-like cell clusters and differentiated into neuronal lineages in vitro.
|
| Free Research Field |
再生医療
|
