2016 Fiscal Year Research-status Report
プルキンエ細胞樹状突起の情報処理におけるイオンチャネル分布のはたす機能的役割
| Project/Area Number |
15K06725
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| Research Institution | Okinawa Institute of Science and Technology Graduate University |
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Principal Investigator |
洪 聖昊 沖縄科学技術大学院大学, 計算脳科学ユニット, 研究員 (90600205)
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| Co-Investigator(Kenkyū-buntansha) |
高嶋 聰 沖縄科学技術大学院大学, 計算脳科学ユニット, 研究員 (00374190) [Withdrawn]
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | Dendrite / Neuron / Morphology / Computational model / Simulation / Ion channel |
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| Outline of Annual Research Achievements |
- We refined and improved the algorithm of identifying subunits in a dendritic tree of a neuron. We implemented improved clustering algorithms such as a multi-scale spectral clusetring alorithm (Myung et al., J Neurosci, 2012) and a test for clustering based on cluster stability (Lange et al., Neural Comput, 2010).
- We implemented a computer model of an intermediate conductance calcium-activated potassium channel, KCa3.1, experessed in the cerebellar Purkinje cell. The KCa3.1 channel has been reported to play an important role in dendrtic signal processeing in the Purkinje cell (Engbers, PNAS, 2012) while our previous model lacked this channel. Based on the kinetics data of human KCa3.1 channels (Bailey et al, J Gen Physiology, 2010), we built the computer model that reproduced Bailey et al.’s results.
- The parallelized version of the STEPS simulator, which will be used in FY2017, has been developed (Chen and De Schutter, Front Neuroinform, 2017).
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| Current Status of Research Progress |
Current Status of Research Progress
3: Progress in research has been slightly delayed.
Reason
Since our former co-PI Dr. Akira Takashima left the OIST and research team, our progress in modeling, particularly using the NEURON simulator, is significantly impacted. We decided to use a new model independently developed by another researcher in our group, and are waiting for completion of the development, which is expected to be done soon. On the other hand, development of the parallel STEPS simulator is on schedule, and we expect to meet our final goal of simulating the model on the STEPS simulator.
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| Strategy for Future Research Activity |
We are currently waiting for the development of the Purkinje cell model running on the NEURON simulator, which will be deterministic without any stochastic element. This year, we will convert this model to the parallel STEPS simulator, which is already ready for use. The STEPS simulator provides much more flexibility and physiologcal precision than the NEURON simulator in simulating distributed and stochastic intracellular processes. We will implement the calcium mediated interaction between calcium and calcium-activated potassium channels in dendrites.
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| Causes of Carryover |
Since our co-PI Dr. Akira Takashima left the research team, we did not purchase a computer for him.
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| Expenditure Plan for Carryover Budget |
We will purchase a more powerful graphical processing unit that will be added to the already purchased computer.
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