2017 Fiscal Year Final Research Report
The roles of the chamorepellent draxin in hippocampal neurogenesis
| Project/Area Number |
15K06733
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2015-10-21 – 2018-03-31
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| Keywords | draxin / DCC / 海馬神経新生 / アポトーシス |
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| Outline of Final Research Achievements |
Hippocampal neurogenesis in the dentate gyrus (DG) is controlled by various diffusible molecules that modulate neurogenic processes. To elucidate the mechanisms underlying hippocampal neurogenesis, we investigated the function of draxin in the regulation of neuronal survival in the DG. Draxin was expressed in Tbr2 (+) late progenitors and NeuroD1 (+) neuroblasts in the dentate granule cell lineage, whereas expression of its receptor DCC was mainly detectable in neuroblasts. Our phenotypic analysis revealed that draxin deficiency led to enhanced apoptosis of DCC-expressing neuroblasts in the neurogenic areas. Furthermore, in vitro assays using a hippocampal neural stem/progenitor cell (HNSPC) line indicated that draxin inhibited apoptosis in differentiating HNSPCs, which express DCC. Taken together, we postulate that draxin plays a pivotal role in postnatal DG neurogenesis as a dependence receptor ligand for DCC to maintain and promote survival of neuroblasts.
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| Free Research Field |
神経発生学
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