Analyses for synapse-nuclear signaling via a PSD-localizing transcription factor

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K06771
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: Shinshu University
• Principal Investigator: Suzuki Tatsuo 信州大学, 医学部, 特任教授 (80162965)
• Co-Investigator(Kenkyū-buntansha): 白井 良憲 信州大学, 学術研究院医学系, 助教 (70342798)
• Research Collaborator: Li Weidong 上海交通大学, バイオX研究院, 教授
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: postsynaptic density / local protein synthesis / splice variants / synaptic plasticity / transcription factor / mRNA / Gtf2i / dendrite

Outline of Final Research Achievements

We identified a Gtf2i splice variant mRNA that is specifically localized at the postsynaptic sites. We also identified in the brain four proteins that interact with a limitted sequence in the 5'UTR of postsynaptically localizing Gtf2i mRNA. Then we studied mechanism(s) for regulation of local protein synthesis of the Gtf2i protein at the postsynaptic sites. Our experiment suggests that expression of postsynaptically localized Gtf2i proteins are under control of multiple protein factors at the postsynaptic site via interaction with 5'UTR of postsynaptically localizing Gtf2i mRNA.

Free Research Field

神経科学