Analysis of molecular network regulating spatiotemporal activities of three G proteins that control membrane traffic during outgrowth of growth cones

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K06782
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: Tokyo University of Science
• Principal Investigator: NAKAMURA Takeshi 東京理科大学, 研究推進機構生命医科学研究所, 教授 (60362604)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: FRET / Rab GTPase / Rho GTPase / neurite outgrowth / regeneration / sensor / membrane traffic

Outline of Final Research Achievements

In neuronal cells, down-regulation of TC10 and RhoA activities is executed by cAMP-PKA-STEF-Rac1-p190B pathway and leads to neurite outgrowth through membrane addition and cytoskeletal reorganization. In TC10 KO mice, neurite outgrowth during development was not largely different between wild type and KO mice,; however, axon regeneration capability of adult KO mice was significantly lower than that of wild type ones. My group searched the design structure of Rab11 sensor more widely and developed the sensor with dynamic range of about 40%. We investigated Rab11 activity on vesicles that move in neurites. We fabricated the Rab35 sensor having dynamic range of about 50%, examined it in the PC12 response to NGF etc, and confirmed that the sensor has nearly the prescribed function.

Free Research Field

分子・細胞神経科学