Elucidation of pathogenesis of diabetes in the novel obese diabetes model ZFDM rat

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K06800
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory animal science
• Research Institution: Kobe University
• Principal Investigator: YOKOI NORIHIDE 神戸大学, 医学研究科, 特命准教授 (70311610)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 糖尿病 / 疾患モデル / インスリン分泌障害 / メタボローム解析 / 病態発症機構

Outline of Final Research Achievements

In this study, we tried to elucidate the pathogenesis of diabetes in the novel animal model of obese type 2 diabetes, the Zucker fatty diabetes mellitus (ZFDM) rat. By insulin secretion experiment, transcriptome analysis, metabolome analysis, and western blotting of isolated pancreatic islets, we found that the number of large islets increased with age and insulin secretion was impaired in the large islets. We also found that glycolysis and hexosamine biosynthesis pathways were enhanced and downstream O-GlcNAcylation of proteins was increased in the large islets. These findings may underlie the elucidation of pathogenesis of diabetes and development of drugs based on the novel mechanism.

Free Research Field

分子代謝学、実験動物学