2017 Fiscal Year Final Research Report
Analysis of Inv gene functions by generation of novel transgenic mice.
| Project/Area Number |
15K06808
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | Kitasato University |
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Principal Investigator |
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| Research Collaborator |
MENO Chikara
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | Inv遺伝子 / 左右軸形成 / 多発性嚢胞腎 / コンディショナルノックアウト / コンディシショナルレスキュー / トランスジェニックマウス |
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| Outline of Final Research Achievements |
The Inv gene is an important gene involved in the left/ right axis determination in the development of animals. It is also known to be a causative gene of nephronophthisis type 2 (NPHP2)in human. In this study, to analyze the physiological function of the Inv gene, we devised a conditional rescue technic to induce specific genes inactivation in arbitrary tissues and we succeeded in making conditional rescue mice capable of Inv gene inactivation in tissue-dependent manner. The transgenic mice produced in this study will be used for elucidating the mechanism of the left/right axis determination of animals and the basic research for treating human kidney disease. In addition, the conditional rescue technic developed in this study is expected to be widely applied as a new analytical method of gene function in the future.
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| Free Research Field |
分子発生学
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