Development of simple preservation and transportation method for mouse embryos and spermatozoa without liquid nitrogen and dry ice

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K06816
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory animal science
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Mochida Keiji 国立研究開発法人理化学研究所, バイオリソースセンター, 専任技師 (60312287)
• Research Collaborator: Hasegawa Ayumi 国立研究開発法人理化学研究所, バイオリソースセンター, テクニカルスタッフⅡ ; Kito Seiji 国立大学法人名古屋大学, 動物実験支援センター・東山動物実験施設, 施設長
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 凍結保存 / 冷蔵保存 / 胚 / 精子 / マウス / 輸送

Outline of Final Research Achievements

We established the conditions of simple preservation method for mouse embryos and spermatozoa without liquid nitrogen and dry ice at -40℃ and -2℃. Then, we established the conditions and methods of preservation at temperature ranges from -5℃ to 37℃. Especially, we succeeded to obtaining healthy offspring from both embryos which were stored in refrigerator for 7 days or transported using a commercial service at refrigerated temperature within 5 days. By these technical development, the expense of the transportation for mouse embryos from Japan to Europe or United States of America can reduce to 1/10 - 1/20. Additionally, we succeeded in choosing two candidate genes about cryopreservation sensitivity of the sperm as a more progressive study. Finally, we could establish several new technologies for practical use and mechanism analysis of the cryopreservation and the refrigeration transportation by this funded project.

Free Research Field

実験動物学