Investigation for the superiority of collective cell movement upon the distant metastasis

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K06822
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Tumor biology
• Research Institution: Akita University
• Principal Investigator: Kuriyama Sei 秋田大学, 医学(系)研究科(研究院), 准教授 (30398226)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 浸潤・転移

Outline of Final Research Achievements

Loss of cell-cell adhesion from epithelial cancer often promotes infiltration and metastasis. In our previous report, the modified lung cancer cells, which has stable cell-cell adhesions, actually promote the dissemination to the opposite lungs. In this study, we planned to clarify how the changes of cell-cell adhesion properties affected on the ability of the distant metastasis. The sub-cell lines experimentally induced metastatic characteristics to liver and kidney changes various cell adhesion molecules. Also gene profiling of these sub-cell lines and the parental cells revealed that PEDF gene was upregulated, which was formerly expected as the anti-cancer drug. We made the parental cell line overexpressing PEDF, and tested gene expression, cell migration, and in vivo metastasis. The cells injected into knee joint produced the primary lesions orthotopically, and metastasized to liver and kidney too. We further researched the unexpected mechanism about PEDF-induced metastasis.

Free Research Field

腫瘍生物学