Changes in the binding regions of Smads downstream of TGF-beta signaling in diffuse type gastric cancers

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K06831
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Tumor biology
• Research Institution: The University of Tokyo
• Principal Investigator: Koinuma Daizo 東京大学, 大学院医学系研究科(医学部), 准教授 (80375071)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: シグナル伝達 / 癌 / 転写因子 / TGF-beta / ChIP-seq / スキルス胃がん

Outline of Final Research Achievements

Cytokines of transfroming growth factor-beta (TGF-beta) play important roles in the pathogenesis of diffuse type gastric cancers. We previously reported that TGF-beta and the binding sites of its downstream Smad2/3 transcription factors are deferentially regulated in different types of the cells. We here analyzed our ChIP-seq data of Smad2/3 obtained from several diffuse type gastric cancer cell lines to reveal its regulatory mechanisms. Through comparison with the Smad2/3 binding data of the other cells, we identified a target gene of TGF-beta whose expression was deferentially correlated with the prognosis of patients with gastric cancer. We also found that this target gene regulated cellular motility, suggesting its roles in the progression of cancers.

Free Research Field

分子病理学・シグナル伝達・癌