Basic Analysis for internal radiation therapy using stem cell

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K06868
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Tumor therapeutics
• Research Institution: The University of Tokyo
• Principal Investigator: Nomura Sachiyo 東京大学, 医学部附属病院, 准教授 (70301819)
• Co-Investigator(Kenkyū-buntansha): 細谷 紀子 東京大学, 大学院医学系研究科(医学部), 講師 (00396748)
• Co-Investigator(Renkei-kenkyūsha): MOMOSE Toshimitsu 東京大学, 医学部附属病院, 准教授 (20219992)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 胃癌 / 腹膜播種 / 内照射 / 間葉系幹細胞 / 放射性ヨード

Outline of Final Research Achievements

We established mouse gastric cancer cell lines that can be transplanted into C57BL/6 mice, and found that cancer associated fibroblast is of bone marrow origin. Gastric cancer peritoneal dissemination model was established with these cell lines. NIS was transfected into bone marrow derived mesenchymal stem cells (BMDC) that were already transfected with SV40T large antigen, and NIS expressing BMDC was established.
CRISPR/CAS9 system was confirmed to use for gene knock-down for the gastric cancer cell lines, and the methods for gathering BMDC around the peritoneal disseminated cancer cells were analyzed. And GFP expressing BMDC was used and the way to gather BMDC efficiently around peritoneal disseminated gastric cancer cells were established.

Free Research Field

消化器癌治療