2017 Fiscal Year Final Research Report
Development of in vitro enChIP and its applications to analysis of molecular mechanisms underlying genome functions
| Project/Area Number |
15K06895
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Genome biology
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
|
| Keywords | in vitro enChIP / enChIP / locus-specific ChIP / ChIP / CRISPR / TAL / chromatin / chromosomal conformation |
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| Outline of Final Research Achievements |
Elucidation of molecular mechanisms underlying genome functions requires identification of molecules interacting with genomic regions of interest in vivo. In this study, we developed in vitro enChIP using purified CRISPR complexes to isolate a target genomic region while retaining molecular interactions. Combining in vitro enChIP with next-generation sequencing analysis, we succeeded in identifying genomic regions that interact with a target locus in vivo. In addition, we showed that an interacting genomic region works as an enhancer to upregulate transcription from the target locus. Thus, in vitro enChIP is a useful tool for elucidation of molecular mechanisms underlying genome functions.
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| Free Research Field |
分子生物学
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