Revealing cancer-cell evolution and intra-tumor heterogeneity by single-cell genome and transcriptome analysis

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K06916
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Medical genome science
• Research Institution: National Cancer Center Japan
• Principal Investigator: Kato Mamoru 国立研究開発法人国立がん研究センター, 研究所, 部門長 (40391916)
• Co-Investigator(Kenkyū-buntansha): 筆宝 義隆 国立研究開発法人国立がん研究センター, 研究所, 客員研究員 (30359632) ; 新井 康仁 国立研究開発法人国立がん研究センター, 研究所, 主任研究員 (80222727)
• Co-Investigator(Renkei-kenkyūsha): SHIBATA Tatsuhiro 国立がん研究センター, がんゲノミクス分野, 分野長 (90311414)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: がんゲノム / 一細胞シークエンス / 腫瘍内多様性

Outline of Final Research Achievements

To reveal heterogeneity dynamics of caner, we sampled single cells in a colorectal cancer mouse model right before, and 1 and 3 months after transplantation, and then used C1 to sequence both single-cell exome and transcriptome. We revealed that the heterogeneity of DNA decreased after transplantation, whereas that of RNA increased. This heterogeneity increase corresponded to the newly generated subpopulations of cancer cells. They had distinct features in markers such as cell proliferation, epithelial-mesenchymal transformation, and stem cell, and we revealed the dynamics of the feature change. We also found associations with metastatic colonization and a certain clinical factor.

Free Research Field

バイオインフォマティクス